|
Volume 19, Issue 2, 2010
LETTER: DEVELOPMENT OF 3D BIOACTIVE NANOCOMPOSITE SCAFFOLDS MADE FROM GELATIN AND NANO BIOACTIVE GLASS FOR BIOMEDICAL
APPLICATIONS
M. Mozafari1,*, F. Moztarzadeh1, M. Rabiee1, M. Azami1, N. Nezafati1, Z. Moztarzadeh2, M. Tahriri1
1 Biomaterials Group, Faculty of Biomedical Engineering (Centre of Excellence), Amirkabir University of Technology, P. O. Box: 15875-4413, Tehran, Iran
2 Institute of Bioinformatic, Münster University, Münster, Germany
* Author to whom correspondence should be addressed, e-mail: mmozafari@aut.ac.ir
Received 8 December 2009; accepted 19 February 2010
ABSTRACT
In this research, macroporous, mechanically competent and bioactive nanocomposite scaffolds have been fab¬ricated from cross-linked gelatine (Gel) and nano bioactive glass (nBG) through layer solvent casting com¬bined with freeze-drying and lamination techniques. This study has developed a new composition to produce a new bioactive nanocomposite which is porous with interconnected microstructure, pore sizes are 200-500 µm, porosity are 72%-86%. Also, we have reported formation of chemical bonds between nBG and Gel for the first time. Finally, the in vitro cytocompatability of the scaffolds was assessed using MTT assay and cell attachment study. Results indicated no sign of toxicity and cells found to be attached to the pore walls offered by the scaf¬folds. These results suggested that the developed nanocomposite scaffold possess the prerequisites for bone tissue engineering scaffolds and it can be used for tissue engineering applications.
KEYWORDS:Nanocomposite; Macroporous structure; Gelatin; Bioactive glass; Osteoblast Cells.
|
